intraperitoneal injection in humans
study on peritoneal cells (PCs) migration to gut may give us some hints27. The peritoneum is a cavity which has been successfully utilized by nephrologists to perform peritoneal dialysis (PD) in patients with renal failure. Female nude mice (Envigo) were anesthetized by intraperitoneal (IP) injection of ketamine/xylazine and immobilized in a stereotactic frame (Stoelting). Kalchenko, V. et al. therapy cannot yet be considered as a standard of care for the first-line treatment of ovarian cancer: a systematic review", "Review of intraperitoneal injection of sodium pentobarbital as a method of euthanasia in laboratory rodents", "Changes in "droplet" fractions from rat kidney cells after intraperitoneal injection of egg white", "Errors Related to Different Techniques of Intraperitoneal Injection in Mice", "Postoperative pain after abdominal hysterectomy: a randomized, double-blind, controlled trial comparing continuous infusion vs patient-controlled intraperitoneal injection of local anaesthetic", "Cannabinoid Disposition After Human Intraperitoneal Use: An Insight Into Intraperitoneal Pharmacokinetic Properties in Metastatic Cancer", "Intraperitoneal Route of Drug Administration: Should it Be Used in Experimental Animal Studies? Zhu, H. et al. In our study, we tracked the GFP+ MSCs colon colonization, but our results also showed the short stay of MSCs after injection. Blonski, W., Buchner, A. M. & Lichtenstein, G. R. Treatment of ulcerative colitis. Stem cell research & therapy 6, 55, doi: 10.1186/s13287-015-0066-5 (2015). FOIA All the tissue sections in this study were scan and analyzed with Virtual slide scanning system (VS120-S, Olympus). In patients with peritoneal surface malignancies, perioperative IP chemotherapy has been used with good results. Google Scholar. CAS Sci. Shamsi M, Sedaghatkish A, Dejam M, Saghafian M, Mohammadi M, Sanati-Nezhad A. The AI and IV showed no significant intergroup difference, though AI had lower score (Fig. Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5(+) stem cell. Sphingosine-1-phosphate links persistent STAT3 activation, chronic intestinal inflammation and development of colitis-associated cancer. However, doubts exist over the use of Fe 3 O 4-nanoparticles in human beings. MSCs organ distribution and engraftment was analyzed and quantified by GFP+ MSCs as well as Near-infrared fluorescence imaging. Collagen deposition limits immune reconstitution in the gut. In addition, from the point of cell morphological appearance, most of MSCs kept intact in IP group, while in AI route, other than active MSCs, cell fragments and debris were also identified in the lumen (Fig. National Library of Medicine Dosage: For intravenous (preferred), intracardial, intrapleural, or intraperitoneal injection. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/, Wang, M., Liang, C., Hu, H. et al. Opioids can produce peripheral analgesic effects by activation of opioid receptors on sensory nerves. Frontiers | Efficacy of intratympanic or postauricular subperiosteal Intraperitoneal Chemotherapy - Memorial Sloan Kettering Cancer Center While this route is an easy to master, quick, suitable for chronic treatments and with low impact of stress on laboratory rodents, there is a common concern that it may not be an acceptable route for drug administration in experimental studies. Once the animal is properly restrained (as described above for SC injections) the injection site is . DailyMed - PIPERACILLIN AND TAZOBACTAM injection, powder, lyophilized Pulmonary passage is a major obstacle for intravenous stem cell delivery: the pulmonary first-pass effect. (A,B) Representative colon images and quantification of colon length; (C) myeloperoxidase (MPO) activity in colonic protein extracts; (D,E) Representative H&E staining and corresponding severity score; (F,G) Representative sirius red staining and quantification of collagen deposition. International journal of molecular medicine 37, 115125, doi: 10.3892/ijmm.2015.2413 (2016). Stem cell reviews 10, 127144, doi: 10.1007/s12015-013-9468-x (2014). The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Our findings suggested that IP delivery showed higher MSCs and better experimental colitis recovery, might be an ideal way for MSCs therapy in IBD. There was a dose-dependent decrease in AGT activity in liver tissues of CD-1 mice to 24% of basal levels after four hourly intraperitoneal injections of O 6-methylguanine (110 mg/kg). Exposure to BHT through inhalation, dermal, and oral routes is harmful. Google Scholar. Parenteral administration routes include, but are not limited to epidermal, intraarterial, intramuscular (IM and depot IM) , intraperitoneal (IP) , intravenous (IV) , intrastemal injection or infusion techniques, intranasal (inhalation) , intrathecal, injection into the stomach, subcutaneous injections (subcutaneous (SQ and depot SQ . However, more studies described MSCs as hit and run therapy, which discovered most of MSCs died and cleared from the body within 48 to 72h20,41,42,43. query serum metabolite genome-wide association study (GWAS) data to identify an association between choline metabolism and feline leukemia virus subgroup C cellular receptor 1 (FLVCR1). 4 The scalp was disinfected and a midline incision was made in the skin. Colitis showed varying degrees of alleviation after three ways of MSCs transplantation and the IP injection showed the highest survival rate of 87.5% and displayed the less weight loss and quick weight gain. Disease recovery was evaluated by histological server score, gross body weight and survival rate. & Correa, D. The MSC: an injury drugstore. In view of these observations, and the fact that NASH is a & Prockop, D. J. Anti-inflammatory protein TSG-6 secreted by activated MSCs attenuates zymosan-induced mouse peritonitis by decreasing TLR2/NF-kappaB signaling in resident macrophages. Histopathological alterations in the mouse brain were detected after SWCNTs exposure. Then, the bone marrow was flushed out with 1mL syringe. There are increasing evidences showed that MSCs mediate their immunomodulatory effects through the induction of regulatory T cells (Treg cells)14. In humans respiratory side-effects were reported in five subjects taking mefloquine, but these cases constituted only 0.1% of studied population (Lobel et al., 2001). They are one of the few ways drugs can be administered through injection, and have uses in research involving animals, drug administration to treat ovarian cancers, and much more. Researchers has tried many creative ways to minimize these risks. In consistence with PCR results, IP groups showed higher IL-10 and lower TNF- than other MSCs delivery ways (Fig. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Biological Sciences, China Agricultural University, Yuanmingyuan West Road No. This site needs JavaScript to work properly. For this reason, more MSCs aggregates would form for IP route and produce higher level of serum TSG-6 under TNF- activation, which help reducing the colon inflammation. Two early reports aroused the global interests of stem cell transplantation in IBD management. 2A), which guarantee the less interference from the background signal that coming from free dye. Fluorescent intensity was quantified and processed using the Living Image Software (Version 4.2; Caliper Life Science). Adv Exp Med Biol 371A, 6165 (1995). PubMed [8], A good example of how intraperitoneal injections work is depicted through "The distribution of salicylate in mouse tissues after intraperitoneal injection" because it includes information on how a drug can travel to the blood, liver, brain, kidney, heart, spleen, diaphragm, and skeletal muscle once it has been injected intraperitoneally.[9]. Intraperitoneal injection (IP), Intravenous injection (IV) or anal injection (AI)? Muller-Ehmsen, J. All reactions were performed in triplicated. PDF EUTHANASIA REFERENCE MANUAL - HumanePro Metabolite tracing, radioactive uptake experiments, and CRISPR screens demonstrate that FLVCR1 is the major plasma-membrane choline transporter. This study goes over the dosages, adverse effects, and more of using intraperitoneal injections of anesthesia.[18]. Clinical Announcement: Intraperitoneal chemo-therapy for ovarian cancer. Growth hormone . [16] This study shows how intraperitoneal injections can be more effective and beneficial than other traditional routes of administration. From the overall appearance, the hyperemia and edema of the colon in all MSCs treatment groups became milder than the DSS control. In contrast, GFP+ cells were showed in the lumen for AI group and lamina propria for IV group (Fig. Though IP injection showed benefits in many human and animal . It can significant reduce failure puncture and associated organ damages caused by blind needle puncturing. M.W., C.L. Li, C. Y. et al. In addition, IP showed more intense Ki-67 stain in bottom of the crypt, which suggested more active mucosa repair proliferation (Fig. This review discusses the usefulness of IP drug therapy and the factors influencing it, as well as strategies to increase the efficacy, and conclude that IP route is an alternate route to the more conventional drug delivery routes, and can be successfully used when the target is within the peritoneal cavity or adjacent tissue. Changing Crohns disease management: need for new goals and indices to prevent disability and improve quality of life. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Half of the volume of your D-luciferin substrate can be injected on one side of the animal and the other half of the volume on the opposite side. Intraperitoneal injection or IP injection is the injection of a substance into the peritoneum (body cavity). Article IP injected human MSC rapidly formed aggregates with mouse macrophages and B220+ lymphocytes and attached to the walls of the peritoneal cavity. Cell stem cell 4, 206216, doi: 10.1016/j.stem.2009.02.001 (2009). Models should begin with local tissue infection, so that bacterial . Injections Intraperitoneal (IP) "Mouse" Once animal is restrained turn over so abdomen is exposed. L.Z. Intraperitoneal Drug Delivery for Ovarian Cancer: Why, How, Who, What MSCs has been found to directly differentiate into end-stage cells to augment bone damages and repair cartilage defects38,39.
